Journal: BMC Infectious Diseases
Article Title: Identification of moa A3 gene in patient isolates of Mycobacterium tuberculosis in Kerala, which is absent in M. tuberculosis H37Rv and H37Ra
doi: 10.1186/1471-2334-5-81
Figure Lengend Snippet: PCR of clinical isolates of M. tuberculosis for moa A3 gene. Lane 1: Marker- λ DNA double digest ( Eco R I/ Hind III), lane 2: H37Rv, lane 3: H37Ra, lane 4: M. bovis BCG, lanes 5–24: Clinical isolates RGTB 29, 37, 40, 43, 55, 60, 70, 86, 87, 93, 95, 109, 110, 123, 142, 144, 154, 167, 177, 193 respectively, lane 25: Negative Control.
Article Snippet: Genomic DNA from M. tuberculosis H37Rv, H37Ra, M. bovis BCG and the clinical isolates digested using Eco R I (New England Biolabs) was separated on agarose gels, transferred to nylon membrane (Hybond) and probed with α 32 P [dCTP] labelled PCR product.
Techniques: Marker, Negative Control